Cultivate liquid mycelium

Table of Contents

liquidmycelium jar

To cultivate liquid mycelium, follow these steps:

  • Sterilize Equipment: Sterilize all equipment, including jars, syringes, and needles, to prevent contamination.

  • Prepare Nutrient Broth: Create a nutrient-rich broth using ingredients like malt extract or potato dextrose. Boil and then cool it.

  • Inoculation: Introduce mycelium spores or tissue into the broth using a sterilized syringe.

  • Incubation: Place the inoculated jars in a dark, warm area. Ideal temperatures are typically around 25°C (77°F).

  • Agitation: Regularly shake or stir the mixture to promote even growth and prevent clumping.

  • Monitor Growth: Watch for the development of mycelial strands in the liquid. This can take several days to weeks.

  • Use or Store: Once fully grown, the liquid mycelium can be used immediately for inoculating substrates or stored in a refrigerator for short-term use.

Remember, maintaining a sterile environment is crucial to prevent contamination and ensure successful growth.